NeoFlow
NeoFlow is a free alternative to FlowJo that combines flow cytometry FCS file analysis with robust vector illustration tools. It supports an end-to-end workflow, taking you from raw data analysis all the way to publication-ready figures.
Key Features:
- Significantly faster processing speeds compared to other applications
- Intuitive, visual compensation controls
- Simultaneous overlay of multiple plots
- Style copying across plots
- FlowJo workspace (.wsp) file import support
- Full-featured drawing and graphic design capabilities
- Import and export support for PDF, PowerPoint, and Adobe Illustrator files
- Vector object copy-and-paste compatibility with graphing and design software
Disclaimer
This application is provided free of charge. The developer assumes no responsibility or liability for any damages arising out of the use of or inability to use this application. We make no warranties regarding its accuracy, completeness, fitness for a particular purpose, or that it is free of bugs or errors. All use of this application is entirely at your own risk. However, if you have any feature requests or encounter bugs, please feel free to contact the address below. Your feedback will be highly appreciated and considered for future improvements.
Hisashi Arase: arase@biken.osaka-u.ac.jp
Citation Request
If you use this application for academic papers or related publications, we kindly request that you cite the application name and this website as the source.
NeoFlow
NeoFlow is a free alternative to FlowJo that combines flow cytometry FCS file analysis with robust vector illustration tools. It supports an end-to-end workflow, taking you from raw data analysis all the way to publication-ready figures.
Key Features:
- Significantly faster processing speeds compared to other applications
- Intuitive, visual compensation controls
- Simultaneous overlay of multiple plots
- Style copying across plots
- FlowJo workspace (.wsp) file import support
- Full-featured drawing and graphic design capabilities
- Import and export support for PDF, PowerPoint, and Adobe Illustrator files
- Vector object copy-and-paste compatibility with graphing and design software
Disclaimer
This application is provided free of charge. The developer assumes no responsibility or liability for any damages arising out of the use of or inability to use this application. We make no warranties regarding its accuracy, completeness, fitness for a particular purpose, or that it is free of bugs or errors. All use of this application is entirely at your own risk. However, if you have any feature requests or encounter bugs, please feel free to contact the address below. Your feedback will be highly appreciated and considered for future improvements.
Hisashi Arase: arase@biken.osaka-u.ac.jp
Citation Request
If you use this application for academic papers or related publications, we kindly request that you cite the application name and this website as the source.

